. Blood 122, e23e32, doi: 10.1182/blood-2013-04-495119 (2013). Veronesi, F. et al. Mice were under intensive observation for 12 days. Stem cells and development 21, 21892203, doi: 10.1089/scd.2011.0674 (2012). Two early reports aroused the global interests of stem cell transplantation in IBD management. Frontiers | Efficacy of intratympanic or postauricular subperiosteal At 24h after injection, the intensity of free dye was 100 folds less than the DiR labeled cells (from the bar value. n=68 mice/group; n=3 for the control and naive (health mice) group. Intraperitoneal Injection - an overview | ScienceDirect Topics Kean, T. J., Lin, P., Caplan, A. I. Clinical Ovarian and Other Gynecologic Cancer 5, 2426, doi: 10.1016/j.cloc.2011.08.001 (2012). Would you like email updates of new search results? The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion. The optimum route of administration is an importance clinical issue not only for chemical drug, but also for stem cytotherapy. Nature medicine 5, 309313, doi: 10.1038/6529 (1999). The authors declare no competing financial interests. and H.H. In quantification study, the intensity of engrafted cells in IP and AI groups were significant higher than the IV (P=0.004, P=0.0012; Fig. A., Ong, J. F. & Karp, J. M. Mesenchymal stem cells: immune evasive, not immune privileged. The IP injection significantly inhibited collagen deposition compared with DSS control (P=0.0008) and IV group (P=0.0139). Second concerning is about the cell dose. Gynecol Oncol 81, 7781, doi: 10.1006/gyno.2000.6108 (2001). Cell and tissue research 347, 419427, doi: 10.1007/s00441-011-1306-3 (2012). In this study, the therapeutic efficiency of three different MSCs delivery routes (IP, AI and IV) were compared on DSS-induced colitis mouse model. In most experimental studies, the MSCs treatment usually require at least 1106 cells/mouse, more frequently, a dose as high as 5106 cells/mouse to observe any effect12. Intraperitoneal administration allows quick reabsorption of large volumes of substances and is the preferred injection route for nonirritant, isotonic solutions [7,11]. Upon intravenous and intraperitoneal injection of stressed MSA, fluorescent "hotspots" were observed in the spleens, livers and lungs. Note where each injection is made and subsequent injections should be made in slightly different spot than the previous injection. Intraperitoneal - an overview | ScienceDirect Topics [8], A good example of how intraperitoneal injections work is depicted through "The distribution of salicylate in mouse tissues after intraperitoneal injection" because it includes information on how a drug can travel to the blood, liver, brain, kidney, heart, spleen, diaphragm, and skeletal muscle once it has been injected intraperitoneally.[9]. Stem cells (Dayton, Ohio) 24, 386398, doi: 10.1634/stemcells.2005-0008 (2006). & Prockop, D. J. Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-kappaB signaling in resident macrophages. There are a variety of uses and possibilities for many more in the future with further research and approval. Cell stem cell 5, 5463, doi: 10.1016/j.stem.2009.05.003 (2009). Growth hormone Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. Three days after MSCs transplatation, the mice were sacrificed for histological evaluation (H&E and phosphomolibidic acid-picrosirius red staining). The delivery of OK-432 occurred a total of four times in a span of one week. The latter is . And the loss of MSCs in the colon may attribute to many reasons, such as, wash out, cell death, or even rejection via the innate immune system. Fluorescent intensity was quantified and processed using the Living Image Software (Version 4.2; Caliper Life Science). 1B). PubMed In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Firstly, peritoneal cavity provides an isolated, hemodynamically stable, sterile and nutritious environment with enough growth spaces, which are very critical factors for aggregates forming. Tyndall, A. The data showed that the TSG-6 levels increased with time in both IP and IV injection groups, while in DSS control group, the TSG-6 level decreased from 48h to 72h. Although there is no significant difference among three injection groups at 48h after MSCs transplantation, the serum TSG-6 was significant higher in IP injection group compared with naive group (P=0.024). Importantly, we identified different migration fates of MSCs and traced their ultimate location of three administration ways for the first time. Article In the meanwhile, the colonic homogenates were collected and colonic IL-10, TNF- were measured by ELISA assays. Comparison of colonic FoxP3 expression 7 days after MSCs injection. Kenny et al. This case presents the question of how THC is absorbed in the peritoneal cavity. Evid Based Complement Alternat Med. Accessibility Gastroenterology 114, 433440 (1998). Further study demonstrated TSG-6 alone was sufficient to reduce intestinal inflammation in mice with colitis. There are few examples of the use of intraperitoneal injections in humans cited in literature because it is mainly used to study the effects of drugs in mice. We believe with the increasing interests for IP injection in the field of cytotherapy, more innovation and creative ideas for reducing IP complications will come up. But how these miracle cells reach to the lumen and by which possible migration route? These findings may benefit clinic practice. The expression level of TNF-, the most important pro-inflammatory cytokine, was markedly decreased in IP group, compared with AI (P=0.0174), IV (P=0.0019) and control group (P<0.001), respectively. Google Scholar. The formation of the aggregates probably limits access of the cells to the systemic circulation. government site. Nonmyeloablative stem cell therapy enhances microcirculation and tissue regeneration in murine inflammatory bowel disease.
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